Team: PIs | |||||
Inputs | Activities | Outputs | Short-term Outcomes | Long-term Outcomes | Impact |
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Germplasm assembly (Objective 1): Leslie, Aradhya, Preece, Hackett
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Walnut germplasm, including mother trees and pollen of diverse Juglans spp. | Generate, propagate, and curate open-pollinated (OP) and hybrid seedlings and clones. Coordinate w/ phenotyping and genetic mapping teams | OP and interspecific hybrid seedling and clonal plants; publications describing propagation technologies | Utilization of germplasm for disease resistance evaluations; development of rootstock breeding populations and technologies | Commercial availability of clonal rootstock selections; nursery adoption of clonal rootstock propagation technologies | Walnut nursery and orchard industries stimulated by new propagation technologies and superior rootstocks |
Phenotyping (Objective 1): Browne, Baumgartner, McKenry, Kluepfel, Coggeshall, Schlarbaum
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OP and interspecific hybrid seedling and clonal plants; preliminary data on disease resistance | Evaluate resistance of the walnut rootstock germplasm to soilborne pathogens; communicate data | Quantitative data and publications on resistance of walnut rootstock germplasm to pathogens | SNP linkage mapping for disease resistance is facilitated; clones with putative resistance to pathogens are produced | Validated SNP markers for disease resistance; clones with resistance to pathogens established in field trials | Enhanced competitive-ness and efficiency of walnut industry due to improved resistance to key pathogens |
Genetic mapping (Objective 2): Aradhya, Dvorak, Dandekar, Neale
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OP and interspecific hybrid seedling and clonal plants; data on disease resistance of germplasm; English walnut SNP map and genomic information | Create association & biparental maps of disease resistance based on SNP segregation and disease resistance in OP and hybrid populations | Comparative genetic maps of wild spp.; linkage of resistance loci & SNP markers; genetic information for use in breeding; genetic publications & databases | Mapped markers linked to genes conferring disease resistance; comparative linkage maps for examination of other traits in walnut germplasm | Marker assisted selection (MAS) used in rootstock breeding; gene pyramiding; combined resistance to several pathogens in single rootstocks | Genetic information will accelerate development of improved walnut rootstocks and improve breeding options |
Extension and Education (Objective 3): Hasey, Lampinen, Browne, Baumgartner, McKenry, Kluepfel
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Disease resistance information; new clonal rootstocks | Partnering with farm advisors, stakeholders, and WRAP to disseminate new rootstock information; conduct new rootstock orchard demonstration trials | Extension publications and web-based outreach describing new rootstocks and their performance | Stakeholder involvement in rootstock evaluations and stewardship; awareness of genetic/ breeding technologies, environmental benefits | Rootstock performance information from long- term trials; publications summarizing orchard performance of rootstocks and validating resistance to pathogens | Informed grower and nursery adoption of new disease-resistant rootstocks; less dependence on soil fumigation |
Novel/Emerging Diseases (Objective 3): Bostock, Browne, Coggeshall, Hasey, Schlarbaum, Seybold
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Reports of new/emerging diseases from UCCE Farm Advisors and growers |
Orchard surveys to characterize new and emerging threats; assess: 1) host susceptibility to G. morbida, (TCD), 2) host preference of insect vector of G. morbida, 3) Koch’s postulates for PCD
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Publications and newsletter articles assessing the incidence and severity of TCD and PCD in walnut | Broadened grower awareness TCD and PCD and their management; Broadened awareness of Juglans genotypes resistant / tolerant to TCD | Disease management strategies for TCD and PCD | Enhanced resiliency of walnut production due to effective threat management |
Economic Analysis (Objective 4): Klonsky, Browne, Baumgartner, Hasey, McKenry, Kluepfel
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Growth, survival and yield data from replicated orchard rootstock trials; nursery production data | Assemble and analyze actual and estimated cost and income data from industry, nurserymen, growers, and collaborating scientists | Extension publications and database entries summarizing cost-benefit analyses for adoption of clonal walnut rootstocks | Grower interest and initial plantings of new rootstocks will be facilitated by relevant economic analyses | Adoption of new clonal rootstocks with resistance to pathogens; and superior adaptation to new environ-ments | Enhanced economic efficiency and sustainability of walnut nursery and orchard production |
Project Management: Kluepfel
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Project and evaluation plan | Project Manager will coordinate research teams and determine progress towards objectives and short-term outcomes | Objective evaluation to document areas in need of improvement over time | Continued modifications and improvement of project to ensure successful conclusion. | Accomplishment of project objectives; effective delivery of genetic resistance and genetic knowledge | Public and industries will benefit from productive use of Federal grant dollars |