Micropropagation

1. The walnut micropropagation lab employs and trains a diverse team of students

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2. Making tissue culture medium

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3. All tissue culture medium has to be sterilzed in an autoclave

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4. Young shoots or suckers of seedlings or Mother Trees for introduction to culture

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5. Introduction of full-sib hybrid seed to culture by extraction of immature zygotic embryos

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6. Shoots cut to single node pieces and washed before sterilizing in bleach solution

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7. Introducing new material to culture by nodal cuttings

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8. A new nodal cutting beginning to push a bud

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9. New shoot excised from the initial nodal cutting

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10. Beginning to multiply a new shoot recently excised from a nodal cutting

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11. A new genotype just established and now ready to produce shoots for rooting

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12. Micropropagation of full-sib hybrid seed for replicated pathogen resistance testing

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13. Micropropagation of potential Mother Trees from nodal cuttings

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14. In the micropropagation lab we also keep an indoor germplasm collection available to support commercial labs

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15. Micropropagation generates plenty of dishes!

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16. Temperature and humidty controlled fog chamber for rooting micropropagated shoots

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17. Micropropagated shoots rooting in a fog chamber

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18. Filling ConeTainers with potting soil to plant newly rooted micropropagated shoots

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19. Recently planted rooted shoots acclimating in a fog chamber before transfer to a shade bench

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20. Partially acclimated micropropagated plantlets acclimating on a shade bench

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21. Repottting acclimatedclonal plantlets to larger pots for Phytophthora resistance trials

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22. Re-potting micropropagated plantlets for Phytophthora resistance testing

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23. Micropropagated plants to be grown further for crown gall resistance testing

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24. Use of gibberellic acid to elongate micropropagated plants for pathology trials

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25. But - use of gibberellic acid in tissue culture medium inhibited shoot growth

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26. Changing the iron chelate and concentration in the medium improved growth of some genotypes

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